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KMID : 0848120070320040127
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International Journal of Oral Biology
2007 Volume.32 No. 4 p.127 ~ p.133
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Inducing apoptosis by the inhibition of c-myb in oral squamous carcinoma cell line, KB cell
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Lee Jung-Chang
Moon Hyun-Ju
Lee Young-Hee
Jung Ji-Eun
Manju Sharma
Jhee Eun-Chung
Yi Ho-Keun
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Abstract
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Oral squamous cell carcinoma (OSCC) is the most common malignancy and is a major cause of worldwide cancer mortality. The proto-oncogene c-myb plays an important role in regulation of cell growth and differentiation, and it is expressed at high levels in hematopoietic cells and many other types of cancers. However, the function of c-myb is not well known in OSCC. The present study aimed to reveal the function of c-myb and to test the alternation of cell growth and signaling by c-myb in OSCC. In this study, c-myb and dominant-negatibe myb(DNmyb) were expressed in an adenovirus-mediated gene delivery system to KB cells. The over-expressed c-myb brought increased cellular proliferation compared with control cells. However, DN-myb infected KB cells showed significant reduction of cell growth and enhanced induction of apoptosis to activate PARP and caspase 9. c-myb induced increase of IGF-I, -II and IGF-IR expressions while DN-myb down-regulated these expression. Activation of ERK and Akt/PKB pathway was shown only in c-myb transduced cells. These findings suggest that the role of c-myb in cell growth of oral cancer cells is partially mediated through the modulation of IGFs, ERK and Akt/PKB. From this results, DN-myb is strongly recommended as a curable gene for the treatment of c-myb dependent malignancies such as OSCC.
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KEYWORD
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Apoptosis, c-myb, Dominant-negative myb, KB cell, Oral squamous cell carcinoma
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